Journal: International Journal of Molecular Medicine
Article Title: Recombinant ADAMTS-1 promotes muscle regeneration accompanied by downregulation of Notch signaling
doi: 10.3892/ijmm.2025.5718
Figure Lengend Snippet: Effect of rADAMTS-1 on MuSCs following BaCl 2 -induced TA muscle injury. Groups included NC, BaCl 2 , and two treatment groups receiving rADAMTS-1 (L) and (H). Daily intraperitoneal injections of rADAMTS-1 were initiated immediately after injury and continued until the designated time point for analysis. (A) Flow cytometry analysis of MuSCs isolated from injured TA muscles, defined by surface marker expression (CD45 − /CD31 − /Sca1 − /VCAM1 + ). (B) Representative gating strategy for MuSCs across experimental groups at different time points. (C) Relative number of MuSCs normalized to the NC group over time. Statistical significance was determined using one-way analysis of variance, followed by Tukey's post hoc test: * P<0.05, ** P<0.01 and *** P<0.001 vs. NC; ## P<0.01 and ### P<0.001 vs. BaCl 2 group. (D) Representative immunofluorescence images of Pax7 + cells in injured TA muscle at different time points (scale bar, 25 μ m). (E) Quantification of Pax7 + cell percentages over time. Statistical significance was determined using one-way analysis of variance with Tukey's post hoc test: * P<0.05 and *** P<0.001 vs. NC; ### P<0.001 vs. BaCl 2 ; ††† P<0.001 vs. rADAMTS-1 (L). ADAMTS-1, a disintegrin and metalloproteinase with thrombospondin motifs 1; rADAMTS-1, recombinant ADAMTS-1; MuSCs, skeletal muscle satellite cells; BaCl 2, barium chloride; TA, tibialis anterior; NC, non-injured control; rADAMTS-1 (L), rADAMTS-1 at 5 mg/kg; rADAMTS-1 (H), rADAMTS-1 at 10 mg/kg; CD, cluster of differentiation; Sca1, stem cell antigen 1; VCAM1, vascular cell adhesion molecule; Pax7, paired box protein.
Article Snippet: Primary skeletal muscle cells (cat. no. PCS-950-010; ATCC) were cultured in a complete expansion medium (cat. no. PCS-500-030; ATCC) using a primary skeletal muscle cell growth kit (cat. no. PCS-950-040; ATCC).
Techniques: Flow Cytometry, Isolation, Muscles, Marker, Expressing, Immunofluorescence, Recombinant, Control